To improve compliance with a gluten-free diet in coeliac disease a simple prototype test kit
was developed to detect gluten in foods for use at home. The test is based on monoclonal
antibodies to heat-stable gluten proteins which crossreact appropriately with barley and rye
proteins. It is suitable for use with a wide range of raw or cooked foods. The food is extracted
with dilute hydrochloric acid and 1 drop of the extract transferred to an antibody-coated tube;
enzyme-labelled gluten detection antibody is added and after 3 min the tube is washed and …